Guanylyl cyclase can't stand the HETE

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Guanylyl cyclase can ’ t stand the HETE 1

Nitric oxide (NO)-sensitive isoforms of heterodimeric guanylyl cyclase (α1β1 and α2β1 8 soluble guanylyl cyclases, sGC) extend an umbrella of protection throughout the 9 cardiovascular system, with wide-ranging effects on local blood flow, platelet & 10 leukocyte reactivity, vessel morphology, and cardiac structure and function(5; 10). 11 Consequently, dysfunctional sGC signalling precipitates ...

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NO activation of guanylyl cyclase.

Nitric oxide (NO)-sensitive guanylyl-cyclase (GC) is the most important receptor for the signaling molecule NO. Activation of the enzyme is brought about by binding of NO to the prosthetic heme group. By monitoring NO-binding and catalytic activity simultaneously, we show that NO activates GC only if the reaction products of the enzyme are present. NO-binding in the absence of the products did ...

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Crystal structure of the guanylyl cyclase Cya2.

Cyclic GMP (cGMP) is an important second messenger in eukaryotes. It is formed by guanylyl cyclases (GCs), members of the nucleotidyl cyclases class III, which also comprises adenylyl cyclases (ACs) from most organisms. To date, no structures of eukaryotic GCs are available, and all bacterial class III proteins were found to be ACs. Here we describe the biochemical and structural characterizati...

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Soluble guanylyl cyclase (sGC) is activated by nitric oxide (NO) and generates the second messenger cyclic GMP (cGMP). Recently, purified sGC α1β1 has been shown to additionally generate the cyclic pyrimidine nucleotides cCMP and cUMP. However, since cyclic pyrimidine nucleotide formation occurred only the presence of Mn(2+) but not Mg(2+), the physiological relevance of these in vitro findings...

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ژورنال

عنوان ژورنال: American Journal of Physiology-Heart and Circulatory Physiology

سال: 2016

ISSN: 0363-6135,1522-1539

DOI: 10.1152/ajpheart.00326.2016